TABLE 4.
RNA library KD efficiency
| Cell line and condition | smg5_depletion | smg6_depletion | smg7_depletion |
|---|---|---|---|
| HEK_NoKO_SMG5-KD_Z023 | 75.8% | ||
| HEK_NoKO_SMG6 + SMG7-KD_Z023 | 17.6% | 69.4% | |
| HEK_SMG7KO_SMG5-KD_Z245 | 77.2% | * | |
| HEK_SMG7KO_SMG5-KD_Z319 | No change | * | |
| HEK_SMG7KO_SMG6-KD_Z245 | 17.6% | * | |
| HEK_SMG7KO_SMG6-KD_Z319 | 32.6% | * | |
| HeLa_NoKO_SMG6 + SMG7-KD_Z021 | n.s. | 53.3% | |
| MCF7_NoKO_SMG6 + SMG7-KD | n.s. | 67% | |
| U2OS_NoKO_SMG6 + SMG7-KD | n.s. | 75.3% |
-
Target depletion is computed as percentage in RNA expression reduction relative to control condition.
(*) Indicates that the SMG7 KO is a point mutation with no change in expression. (n.s.) Not significant.
