Identification of a putative Gag binding site critical for feline immunodeficiency virus genomic RNA packaging

hSHAPE-validated secondary structure models of the first 744 nt of FIV gRNA harboring mutations in U⁸⁵CUG⁸⁸. (A) Mutant AD109i. (B) Mutant AD111i. (C) Mutant AD113i. (D) Mutant AD114i. The reactivity of each nucleotide in the RNA secondary structures is color-coded as indicated in the SHAPE reactivity key. The substitution mutations introduced in the U⁸⁵CUG⁸⁸ motif are highlighted and boxed in red and enlarged as insets. The hSHAPE reactivity data for each mutant is provided in Supplemental Table S2. (PBS) Primer binding site.