Identification of a putative Gag binding site critical for feline immunodeficiency virus genomic RNA packaging

hSHAPE-validated secondary structure model of the first 744 nt of FIV spliced env mRNA (AD27). (A) The reactivity of each nucleotide in the RNA secondary structure is color-coded as indicated in the SHAPE reactivity key. The U⁸⁵CUG⁸⁸ motif is boxed in red, showing the base paired nature of the U⁸⁵CUG⁸⁸ stretch in the spliced env mRNA. (B) Histogram comparing the SHAPE reactivities of nucleotides 80–92 between the WT (AD26) and spliced env mRNA (AD27).