Importance of RNA contacts near PCCRs. (A) For each PCCR, six 50-nt bins centering in the middle of the complementary sequence were chosen, and split read counts of RNA contacts from all RIC-seq experiments were computed for 6 × 6 = 36 combinations. (B) The performance (TPR, true positive rate, vs. FPR, false positive rate) of the random forest classifier predicting the presence of forked eCLIP peaks as a function of spread alone (green), RIC-seq support alone (orange), and spread and RIC-seq support together (blue). AUC is the area under the curve. (C) Feature importance (color map) for 36 bin combinations. The two most important features were the contacts between 1L and −1R and between −1L and 1R, which correspond to the inner and outer contacts immediately adjacent to the PCCR.
