FIGURE 7.
Effect of temperature on glpT expression. (A) Schematic representation of the glpT′-′bgaB translational fusion showing the P and SD sequence. The glpT RNAT is depicted as a lollipop. (B) glpT RNAT. SD sequence is in magenta and three point mutations that stabilize the thermometer are in cyan. (C) β-Galactosidase activity of the glpT′-′bgaB translational fusion grown at the indicated temperatures during exponential and stationary phase growth. Values are averages of at least three independent experiments ± sd. WT, wild type fusion; mut, mutant fusion with the three mutations shown in (B). (D) Same as (C) except the intrinsic terminator was deleted from the glpT leader region.
