FIGURE 6.
Effect of temperature on glpF expression. (A) Schematic representation of the glpF′-′bgaB translational fusion showing the promoter (P) and SD sequence. The glpF RNAT is depicted as a lollipop. (B) glpF RNAT. SD sequence is in magenta and two point mutations that stabilize the thermometer are in cyan. (C) β-Galactosidase activity of the glpF′-′bgaB translational fusion grown at the indicated temperatures during exponential and stationary phase growth. Values are averages of at least three independent experiments ± sd. WT, wild type fusion; mut, mutant fusion with the two mutations shown in (B).
