Reactivity changes with temperature identify potential regulatory RNA elements. (A) Reactivity per nucleotide for the glpF 5′ UTR at 23°C, 30°C, 37°C, and 42°C. Numbering extends from the start of transcription to the start codon. (B) Reactivity for nucleotides 88–150 of the glpF 5′ UTR mapped onto the predicted secondary structure. Normalized reactivity ranges from low (pale orange) to high (dark orange). The position of the SD sequence is shown. (C) Reactivity per nucleotide for the glpT 5′ UTR at 23°C, 30°C, 37°C, and 42°C. Numbering extends from the start of transcription to the start codon. (D) Reactivity for nucleotides 84–158 of the glpT 5′ UTR mapped onto the predicted secondary structure. Normalized reactivity ranges from low (pale orange) to high (dark orange). The position of the SD sequence is shown. The schematics in panels B and D begin immediately downstream from experimentally identified intrinsic terminators and extend to the AUG start codon (shown in lowercase letters).
