Double-stemmed and split structural variants of fluorescent RNA Mango aptamers

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FIGURE 5.
FIGURE 5.

Cotranscriptional fluorescence of unimolecular and split Mango constructs. Fluorescence (left, y-axis) and RNA concentration (right, y-axis) over the course of transcription time (x-axis) for (A) unimolecular dsMango i-iv ii-i, (B) unimolecular Mango I, and (C) split Mango. Fluorescence data represents the mean at that time (n = 3). Error bars on RNA concentration represent CI of 68%. Fluorescence and RNA were separate reactions under identical conditions.

This Article

  1. Published in Advance June 2, 2023, doi: 10.1261/rna.079651.123 RNA 29: 1355-1364

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