Double-stemmed and split structural variants of fluorescent RNA Mango aptamers

Fluorescence of the split dsMango i-iv ii-i (split Mango) construct. (A) Image of fluorescent response for 5′ split Mango strand only, 3′ split Mango strand only, both strands of split Mango, dsMango i-iv ii-i, and Mango I. Color is inverted such that fluorescence appears dark. (B) Abstract representation of split Mango with the 5′ strand (blue) and 3′ strand (yellow) highlighted. (C) Comparison of F_max for split Mango (equimolar 5′,3′) and dsMango i-iv ii-i normalized to Mango I. (D) Fluorescence saturation curves comparing response of Mango I, dsMango i-iv ii-i, and split Mango (equimolar 5′,3′). (E) Fluorescence saturation curves for split Mango where either the 5′ or 3′ are held at 125 nM while the complementary strand is varied. Fluorescence of the split Mango 5′ and 3′ strand alone was also evaluated. Shaded regions and error bars represent 68% CI.