Abstract representations of the dsMango variants. (A) dsMango i-iv ii-i with the first stem in the i-iv position and second stem attachment point at the ii-i corner. Nucleotides of the tetraloop-like motif is highlighted in yellow. These nucleotides were mutated in subsequent experiments. (B) dsMango i-iv iii-ii with the first stem in the i-iv position and second stem attachment point at the iii-ii corner. (C) dsMango i-iv iv-iii with the first stem in the i-iv position and second stem attachment point at the iv-iii corner. (D) dsMango ii-i iii-ii with the first stem in the ii-i position and second stem attachment point at the iii-ii corner. (E) dsMango ii-i iv-iii with the first stem in the ii-i position and second stem attachment point at the iv-iii corner. (F) dsMango iii-ii iv-iii with the first stem in the iii-ii position and second stem attachment point at the iv-iii corner.
