Retained intron transcripts of Gabbr1 and Noc2l are dispersed in the nucleus and partially colocalize with speckles. (A) FISH images of C2C12 cells simultaneously probed for Malat1 (ATTO488, green), Noc2l intron 12 (ATTO647N, red), and Noc2l-exon (ATTO565, blue). DAPI stain is shown in gray. (B) Violin plots showing numbers and distribution of colocalized Noc2l exon and intron spots per cell, and numbers of intron spots colocalized with speckle signal per cell, across 101 cells. (C) Box plots showing the ratios of the colocalized Noc2l intron–exon spots over the total exon spots in nucleus (In-Ex/All-Ex) per cell, and the intron spots with overlapping speckle (Malat1) signal over the total intron spots in nucleus (In-Speckle/All-In) per cell, across 101 cells. (D) FISH images of C2C12 cells simultaneously probed for Malat1 (ATTO488, green), Gabbr1 intron 5 (ATTO647N, red), and Gabbr1 exons (ATTO565, blue). DAPI stain is shown in gray. (E) Violin plots showing numbers and distribution of colocalized Gabbr1 exon and intron spots per cell, and numbers of intron spots colocalized with speckle signal per cell, across 104 cells. (F) Box plots showing the ratio of the colocalized Gabbr1 intron–exon spots over the total exon spots in nucleus per cell, and the intron spots with overlapping speckle (Malat1) signal over the total intron spots in nucleus per cell, across 104 cells. (G) FISH images of C2C12 cells simultaneously probed for Malat1 (ATTO488, green), Gabbr1 intron 5 (ATTO565, red), and Noc2l intron 12 (ATTO647N, blue), with DAPI stain in gray.
