tRNA^Tyr has an unusually short half-life in Trypanosoma brucei

RNAi Knockdown of RRP44 stabilizes both tRNA^Tyr conformers. (A,B) Northern blot hybridization performed on the extracted total RNA samples from RRP44 RNAi strain T. brucei cells subjected to Actinomycin D treatment with and without RRP44 depletion. The tRNA^Glu was used as loading control. (C–F) Linear regression calculations utilizing the amounts of mature and alternate conformer tRNA^Tyr with and without RRP44 depletion. Data were fit to a one-phase decay with R² values as indicated. All data plotted were the result of three biological replicas.