tRNA^Tyr has an unusually short half-life in Trypanosoma brucei

RNAi knockdown of Dis3L2 and RRP6. (A,C) Triplicate growth curve of T. brucei cells subjected to tetracycline-induced protein knockdown where total RNA was collected on day 6, and northern blot hybridization performed on the extracted total RNA samples revealing the mature and alternate conformers of tRNA^Tyr. (B,D) Relative amounts of alternate conformer tRNA^Tyr and mature tRNA^Tyr. The graphs are the result of three biological replicas. The asterisks in D denote significance based on a two-tailed paired t-test with a P-value of 0.03. “ns” stands for nonsignificant by the same test.