tRNA^Tyr has an unusually short half-life in Trypanosoma brucei

RNAi knockdown of XRN enzymes. (A) Northern blot hybridization performed on the extracted total RNA samples revealing the mature and alternate conformers of tRNA^Tyr. The tRNA^Glu was used as loading control. (B–E) Triplicate growth curve of T. brucei cells subjected to tetracycline-induced protein knockdown where total RNA was collected on day 6. Relative amounts of alternate conformer tRNA^Tyr and mature tRNA^Tyr. The graphs are the result of three biological replicas. The asterisks in “E” denote significance based on a two-tailed paired t-test with a P-value of 0.006. “ns” stands for nonsignificant by the same test.