tRNA^Tyr has an unusually short half-life in Trypanosoma brucei

T. brucei tRNA^Tyr has an unusually short half-life, which is not shared by other tRNAs in the system. (A) Triplicate growth curve of WT T. brucei cells subjected to Actinomycin D treatment where total RNA was collected at the time points indicated. (B,C) Linear regression calculations utilizing the amounts of mature and alternate conformer tRNA^Tyr. (D) Linear regression calculations utilizing the amounts of other tRNAs. Data were fit to a one-phase decay. All data plotted were the result of three biological replicas.