T. brucei tRNATyr is present in two conformers. (A) Northern blot hybridization performed on total RNA sample extracted from T. brucei reveals two forms of tRNATyr, including the mature (75 nt) band and a higher (∼120 nt), alternate conformer. (B) Triplicate growth curve of WT T. brucei cells where total RNA was collected at every time point. (C) Northern blot hybridization performed on the extracted total RNA samples revealing the mature and alternate conformers of tRNATyr. The tRNAGlu was used as loading control. (D) Plotted amounts of alternate conformer (alt-tRNATyr) in relation to tRNATyr. (E) Plotted amounts of mature tRNATyr in D and E are the result of at least three biological replicas. Asterisks denote significance as determined by a one-way ANOVA yielding a P-value of 0.0003 for the data in D. “ns” stands for nonsignificant by the same test.
