STAMP does not work well in Drosophila S2 cells. (A) Schematic of the expression constructs used to test TRIBE and STAMP in Drosophila S2 cells. (B) The average number of editing sites identified using Hrp48 and Thor TRIBE (orange) and STAMP (blue) in two biological replicates. ADAR and APOBEC-only controls are also shown to illustrate the background editing of each enzyme (gray). Error bars indicate standard deviation. (C) Editing sites identified in both biological replicates and not found in enzyme-only controls were quantified and normalized relative to the total number of reads in the RNA sequencing library. (D) The number of target transcripts identified by applying TRIBE and STAMP fused to Hrp48 and Thor are indicated.
