TDP-43-ADAR and TDP-43-APOBEC identified common edited regions and transcripts. (A) The coordinates of identified editing sites found in TDP-43-ADAR and TDP-43-APOBEC was expanded by 100 bp in each direction and examined for overlapping regions (>1 bp was considered overlapping). Edited regions found in both TDP-43 TRIBE and TDP-43 STAMP are indicated in blue, and those editing regions that were unique to either TDP-43-ADAR or TDP-43-APOBEC are shown in green. (B) The editing sites identified by TDP-43-APOBEC and TDP-43-ADAR were split into two groups: common sites (those found by both TDP-43-ADAR and TDP-43-APOBEC; blue) or unique sites (found only by one of the editing enzymes; green). These two subsets of editing sites were then overlapped with TDP-43 CLIP sites (any overlap >1 bp was considered overlapping). The percentage of the editing sites overlapping regions containing CLIP peaks is shown. (C) The transcripts identified by TDP-43-ADAR and TDP-43-APOBEC were compared. The graph indicates the percentage of transcripts that are found by both TRIBE and STAMP (blue) or were unique to either TRIBE or STAMP (green). (D) The 100 bp region surrounding RNA editing sites generated by TDP-43-ADAR, TDP-43-APOBEC, and TDP-43-CLIP sites was analyzed for binding motifs using Xstreme (Grant and Bailey 2021). The table indicates the percentage of edited regions that contain GU/GT-rich motifs with high significance.
