Association of SRP9/SRP14 to chromatin is independent of transcription. (A) qPCR analysis of ChIP enrichment of RNA polymerase III, SRP9, SRP14, and an isotype control at the 7SL gene locus in untreated, or 90- min 5 µg/mL actinomycin D treated MCF-7 cells. Data were representative of three independent biological replicates, presented as the mean of three technical replicates and error shown as ±SD. Percent of input was calculated from the measurements of 25 ng input DNA. (B) Same as for (A) but with MCF-7 cells serum deprived for 72 h prior to no treatment (repressed), or addition of 10% serum for 2 h (activated). (C) Same as for (A) but with primers specific to BC200. (D) Same as for (B) but with primers specific to BC200. (E) Same as for (A) but with primers specific to tRNASer(GCT). (F) Same as for (B) but with primers specific to tRNASer(GCT). (G) Relative occupancy comparing the enrichment of each protein after 90 min of actinomycin D treatment relative to untreated. Data were representative of three independent biological replicates, presented as the mean of three technical replicates and error shown as ±SD. (H) Same as for (G) but with relative occupancy comparing enrichment of each protein after addition of 10% serum for 2 h compared to untreated.
