Integrity metrics and examples. (A) Illustration of partially sequenced reads aligned to a reference genome. An example annotation is shown which covers the entirety of the coding region. Note 33.3% of the read's 5′-ends are in the coding region, indicating partial sequencing. (B) Integrity plot comparing the integrity of two S. cerevisiae sequencing data sets from wild-type (WT) and rrp6Δ strains. Two representative examples are shown. (C) IGV screen shot of the reads obtained for SIM1 in the rrp6Δ data set. Most of the reads end within the coding region, resulting in a low integrity score. (D) Same as above for VTC1. Most of the reads do not end within the coding region, resulting in a high integrity score.
