Formamide increases the rate of the reaction between ImpA and pA. (A) Representative HPLC profiles of the reaction between ImpA and its degradation product, pA, to make AppA in either water or 95% formamide over a 4 h time course. (B) Concentrations of ImpA, pA, and AppA predicted (lines) by fitting the reaction scheme in Figure 4 to the HPLC data (points) with a system of ordinary differential equations. The bold lines are the concentrations predicted by the mean of the log-transformed fit rates, and the faint lines are the predicted concentrations from resampling the rates from their 95% confidence intervals. (C) Reaction rates for the scheme in Figure 4 fit by the system of ordinary differential equations.
