Formamide significantly enhances the efficiency of chemical adenylation of RNA sequencing ligation adaptors

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FIGURE 2.
FIGURE 2.

Formamide increases the yield of adenylation across multiple oligonucleotides. (A) A panel of 5′ phosphorylated DNA oligonucleotides were adenylated in either water (W) or 95% formamide (F). (B) Q-TOF LC/MS results of the adenylation reactions confirms the identity of expected products.

This Article

  1. Published in Advance April 14, 2023, doi: 10.1261/rna.079521.122 RNA 29: 1077-1083

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