FIGURE 3.
(A) The anionic G664•G741 pair with some water molecules and a hydrated K+ ion in the deep major groove. (B) The highly conserved organization around the anionic G664•G741 in E. coli (PDB 8B0X, Fromm et al. 2023). The residues G666•U740 form a usual wobble G•U pair. A665/G724 form a cis Hoogsteen/Watson–Crick pair and G722•G733 a trans Watson–Crick/Watson–Crick pair with G722 in the unusual syn conformation. For additional contacts to G664, see Supplemental Figure S2A.
