Initiation of translation on nedicistrovirus and related intergenic region IRESs by their factor-independent binding to the P site of 80S ribosomes

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FIGURE 6.
FIGURE 6.

Mutational analysis of domain 2/PKIII. (A) Influence of APLV1 PKIII deletion on 80S:IRES complex formation, assayed by toeprinting. The influence of disruptive or compensatory substitutions in domain 2 of (B–H) the APLV1 IRES and (I–Q) the NediV IRES on binding of ribosomes, assayed by toeprinting. (A–H) Separation of lanes by white lines indicates that they were juxtaposed from the same gel. Positions of 80S:IRES complexes are indicated on the left (A) or right (C–Q).

This Article

  1. Published in Advance April 11, 2023, doi: 10.1261/rna.079599.123 RNA 29: 1051-1068

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