Deletion of RNAi components affects long-term fitness. (Top) Wild-type and double-knockout strains were grown as more than three lineages and passaged for six generations on AMM agar plates at 37°C for 7 d. Model created with Biorender.com. The evolved strains were then grown for an additional week and the spores were counted using a CASY counter as three biological replicates. The plot shows the values from each biological replicate of each lineage and the mean value of the biological replicates (black line). Significance was determined by one-way ANOVA with Bonferroni-corrected post hoc t-test comparing all columns. (#) P ≤ 0.05, ($) P ≤ 0.01 for all five comparisons with wild-type lineages.
