Single-molecule RNA in situ detection in clinical FFPE tissue sections by vsmCISH

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FIGURE 1.
FIGURE 1.

Implementation of vsmCISH. (A) The workflow of vsmCISH. (B) Detection of HPV-18 E6/E7 expression in HeLa (positive) and A549 (negative) cells. Detection of PGR expression in T47D (positive) and MCF-7 (negative) cells. Scale bar, 50 µm. (C) Statistics plot of the relative frequency of cells without target gene expression.

This Article

  1. RNA 29: 836-846