Duf89 abets lncRNA control of fission yeast phosphate homeostasis via its antagonism of precocious lncRNA transcription termination

Increased duf89-WT or duf89-D252A gene dosage hyperrepresses Pho1 expression. Single colonies (≥20) of wild-type, duf89Δ, and aps1Δ cells bearing pTIN plasmids encoding wild-type or mutant Duf89, or the empty pTIN vector, respectively, were pooled and grown in Leu- ePMG with 15 µM thiamine. Aliquots of exponentially growing cultures were assayed for acid phosphatase activity. The data are averages (±SEM) of three independent biological replicates. Pairwise t-test showed significant differences between aps1Δ cells bearing the empty pTIN plasmid and pTIN-Duf89-WT or pTIN-Duf89-D252A (P-values of 0.019 and 0.027, respectively, denoted by asterisks).