The importance of m6A topology in chicken embryo mRNA: a precise mapping of m6A at the conserved chicken β-actin zipcode

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FIGURE 3.
FIGURE 3.

The RedBaron method of m6A detection: A chimeric oligonucleotide was used to target an RNase H cleavage of phosphodiester bond immediately 3′ to the A/m6A candidate site (A,B). A 32P radiolabeled single-stranded DNA oligonucleotide is ligated to the 3′ of the A/m6A candidate nucleotide (C). The RNA is digested into 3′ nucleoside monophosphates (D). The relative levels of m6A to adenosine are quantified by 2D-TLC (E).

This Article

  1. Published in Advance February 21, 2023, doi: 10.1261/rna.079615.123 RNA 29: 777-789

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