The topology of m6A in the chicken transcriptome. (A) m6A levels in chicken embryo (HH27) and chicken embryonal fibroblast. For each experiment, three biological replicates were used, and the difference between the fibroblast and embryo is not significant (t-test, P = 0.173, one-tailed). (B) mRNA metagene plot from the RNAmod analysis of the MeRIPSeq data from three biological replicates of chicken embryo (HH27). The y-axis represents the density distribution of coverages. (C) Modification sites/peak distribution on different gene features. The y-axis represents the frequency of peaks/sites (number of peaks/sites), whereas the x-axis represents different gene features. The error bars represent the standard deviations for three biological replicates. (D) KEEG pathway enrichment for three replicates shows the top 12 most enriched pathways (data are created using the RNAmod platform, http://61.147.117.195/RNAmod). The color scale represents the enrichment P-value. (E) Integrative Genomics Viewer (IGV) tracks of MeRIP-seq, upper panel and RNA-seq, lower panel read distribution of ACTB mRNA.
