Identification of the dsRNA-associated proteome upon SINV infection in HCT116 cells. (A) Schematic representation of SINV RNA replication and formation of dsRNA intermediates. Capped and polyadenylated viral genomic RNA is represented in black, while viral antigenomic RNA is in green. Gray arrows represent dsRNA replication. ORF1 codes for the nonstructural proteins necessary for RNA replication (in orange). ORF2 codes for the structural proteins (in purple). (B) Schematic representation of the DRIMS experimental approach. (C) Dot blot revealed using the anti-dsRNA J2 antibody on RNA extracted from total lysate (INPUT), dsRNA-IP (J2)- or control-IP (IgG) in mock and SINV-infected HCT116 cells. (D) RT-qPCR on SINV genomic (g)RNA upon dsRNA-IP (J2-IP) compared to control-IP (IgG-IP) on infected samples. Enrichment on three independent IP from three biological replicates are shown. Error bars represent the mean ± standard deviation (SD).
