DNMT1 inhibition by pUG-fold quadruplex RNA

DNMT1 3′UTR binds to DNMT1 but does not inhibit activity in vitro. (A) Binding curves for the WT 3′UTR (purple) and GU mutant (light purple) and GU stretch mutant (pink). (B) Relative DNA methylation in the presence of 1 µM WT 3′UTR (purple) and GU stretch mutant (pink). Methylation levels have been normalized to amount of methylation measured in the “no RNA” (gray) sample. Bars give mean values, with individual data points shown as dots, n = 2.