FIGURE 2.
DNMT1 binds to a variety of RNAs with different affinities in vitro. (A) Schematic of the location of in vitro transcribed RNAs. (B) Representative EMSAs with radiolabeled trace amounts of RNA3 and RNA23 with increasing amounts of DNMT1 protein. (C) Representative EMSAs with radiolabeled trace amounts of (GU)20 and (A)40 with increasing concentrations of DNMT1. (D) Binding curves for all DNMT1 mRNA constructs. (E) Binding curves for all synthetic 40-mer RNA oligonucleotides. In D and E, points represent mean values and error bars represent SD for n = 3 replicates.
