Small noncoding RNA interactome capture reveals pervasive, carbon source–dependent tRNA engagement of yeast glycolytic enzymes

Engagement of GAPDH to tRNAs is carbon source–dependent and regulated by the RNA polymerase III universal repressor Maf1. WT and ΔMaf1 strains were grown in glucose, glycerol and ethanol and UV-cross-linked. Total 2C RNA extractions were performed from two different biological replicates and 20 µg of RNAs were tested on 2C-western blots against GAPDH and ATP1 antibodies. GAPDH* represents residual GAPDH signal after stripping and reprobing the membrane.