GAPDH is enriched in a fraction of short 2C-RNAs. (A) Partition of 2C RNA based on RNA length. 2C RNA from a first extraction was subjected to a second round of 2C under conditions that retain total RNA (upper bioanalyzer electropherogram), RNA molecules longer than 200 nt (middle electropherogram) or RNA molecules shorter than 200 nt (lower electropherogram). (B) Bioanalyzer electropherogram of 2C short RNA loaded on a short RNA chip. (C) 2C RNA from the long and short fractions from CL and NoCL samples were assayed by 2C-western blot and probed against Pab1 and GAPDH antibodies. RNA loaded for the long and short fractions was 15 and 1.4 µg, respectively. GAPDH–RNA complexes are highlighted in the red box.
