FIGURE 5.
AGO2 controls for seed binding. (A) Normalized reactivity of unbound (gray), RNA:RNA (red), and RNA:RNA-RBP complexes (blue) for scrm-seed and the respective heatmaps (B), with the nucleotides opposing the miR-34a seed in green letters/underlined. (C) Secondary structure of the lowest energy conformation of scrm-seed binding to miR-34a predicted with RABS-restrained MC-Fold (Parisien and Major 2008). The normalized reactivity shows different binding patterns for RNA:RNA versus RNA:RNA-RBP, indicating that miR-34a binds the 3′-compensatory region of the scrm-seed, while miR-34a loaded AGO2 does not.
