RNA:RNA interaction in ternary complexes resolved by chemical probing

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FIGURE 2.
FIGURE 2.

Scrambled sequence, the negative control for nonspecific binding. (A) Predicted secondary structures of unbound miR-34a (pink) and unbound scrambled control (scrm, black) using MC-Fold (23). (B) Normalized reactivity of the scrm control inserted in the unbound trans-scaffold (gray), bound by miR-34a (red) and miR-34a loaded AGO2 with respective heatmaps in C, indicating that no unspecific interactions with the RNA or the RNA-RBP complex occur.

This Article

  1. Published in Advance December 20, 2022, doi: 10.1261/rna.079190.122 RNA 29: 317-329

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