FIGURE 1.
The RNA:RNA binding by SHAPE (RABS) approach. (A) The RABS workflow starting from the RNA scaffold design until the interpretation of the binding based on the data obtained. (B) Trans-scaffold design containing the mSIRT1 sequence, and (C) the cis-scaffold containing mSIRT1 and miR-34a sequences. In B and C, the seed region in mSIRT1 sequence and in mSIRT1:miR-34a, respectively, are between green dotted lines. (D) Overview of the three different experimental conditions that we used to evaluate miR:mRNA binding with the trans-scaffold (target sequence in light blue, miR in pink and AGO in light green).
