Human RNase P: overview of a ribonuclease of interrelated molecular networks and gene-targeting systems

RNase P substrates and EGS technology. (A) Representation of a natural substrate (ptRNA). (B–D) A hybridized complex of a target RNA (e.g., mRNA) and an EGS that resembles part of the structure of a tRNA and can be cleaved by RNase P. (C) results from B by deleting the anticodon domain of the EGS, which is dispensable for EGS targeting activity. (D) represents an alternative design based on C. The arrow shows the site of the cleavage by RNase P and RNase P catalytic RNA.