A selective and sensitive detection system for 4-thiouridine modification in RNA

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FIGURE 6.
FIGURE 6.

(A) Schematic drawing of detection of newly transcribed RNAs in S. cerevisiae cells by the MTSEA biotin-XX detection system. (B) Total RNAs from the samples without addition of s4U (left lane)- and with addition of s4U (right lane) were separated by 10% PAGE (7 M urea). The gel was stained with methylene blue. 4-Thiouridine in total RNAs was detected by the MTSEA biotin-XX system.

This Article

  1. Published in Advance November 21, 2022, doi: 10.1261/rna.079445.122 RNA 29: 241-251

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