A selective and sensitive detection system for 4-thiouridine modification in RNA

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FIGURE 1.
FIGURE 1.

(A) Structure of MTSEA biotin-XX. (B) Outline of MTSEA biotin-XX s4U detection system. RNA containing s4U is reacted with MTSEA biotin-XX and fixed onto a nylon membrane filter. Streptavidin-HRP captures the biotin in the sample. The activity of HRP is detected by chemiluminescence. Details are described in the main text and the Material and Methods section. (C) Schematic drawing of MTSEA biotin-XX s4U detection system.

This Article

  1. Published in Advance November 21, 2022, doi: 10.1261/rna.079445.122 RNA 29: 241-251

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