Effects of KRGG1 mutations on RESC association. Anti-RESC13 immunoprecipitations (IPs) co-isolate KRGG1 that is detected via a carboxy-terminal V5-epitope tag. (A) Western analyses of anti-RESC13 IPs (top panel) probed for KRGG1 show stronger signal for WT and functional ARR → AAA mutants relative to dysfunctional mutants in both short and long exposures; probing for either RESC1 or RESC13 shows the isolation of RESCs. Western analyses of cleared lysate inputs used for IPs (bottom panel) probed for the amounts of KRGG1, RESC13, or mitochondrial HSP70 control present in each sample. (B) Densitometric quantitation of the above western blots, with KRGG1 signal in IP (long exposure) normalized to either KRGG1 signal in input or RESC13 signal in IP, RESC1, and RESC13 signal in IP normalized to HSP70 signal in input, and all reported relative to KRGG1 WT sample.
