Effects of KRGG1 mutations on cell growth. (A) The Log2 ratio of cumulative growth without tet versus with tet is shown as a heat map for each day over a 7 d period. The associated scale indicates greater growth inhibition by darker shades of blue, no effect on growth by white and increased growth by darker shades or orange. Controls are the parental conditional null (CN) cell line (top row), cells exclusively expressing a wild-type (WT) KRGG1 (second row), cells with an introduction of the mutant gamma ATP synthase allele (MGA) which permits normal growth in the absence of KRGG1 (third row). Numbering for amino acid substitutions is from the Tb927.6.2230 reference sequence. (B) Location of amino acids that are critical for KRGG1 function in a portion of the Alphafold predicted ARM/HEAT-like tandem helical repeats. The top panel highlights the location of the ARR triplet, and the bottom panel highlights the LLLN region. Amino acids in these regions that are critical for function are noted. Five alpha helices in this region are labeled H1–H5 in amino- to carboxy-terminal order.
