RT-PCR analyses showing the impact of KRGG1 loss on RNA editing in vivo. (A) RT-qPCR assay of BF KRGG1 CN cells showing that over 3 d repression of KRGG1 results in increases of edited ND8 amplicon and decreases of edited A6 amplicon, relative to the telomerase reverse transcriptase (TERT) mRNA internal control. Never-edited, pre-edited, and edited mRNAs are indicated by brackets. (B) RT-qPCR assay as in A but examined over 8 d in BF KRGG1 CN + MGA cells. (C) RT-PCR products corresponding to full length pre-, partially and fully edited ND8, ND3, MURF2, and A6 mRNAs from cells in which KRGG1 is expressed (+) or repressed (−). The arrowheads indicate bands where there were noticeable differences in the ND8 and A6 profiles between KRGG1 expressed versus repressed. Brackets indicate the expected size ranges from pre-edited to fully edited amplicons as noted above for each mRNA.
