FIGURE 2.
KRGG1 is essential for BF growth in vitro. (A) Growth of BF KRGG1 CN that express KRGG1-TAP in the presence of tet (black) and in which KRGG1 expression is repressed upon removal of tet (red). (Inset) Western analysis of KRGG1-TAP cell lysates from day 1 and 2, showing tet-regulation of KRGG1-TAP expression. (B) Growth of BF KRGG1 CN cells expressing mutant gamma ATP synthase allele (MGA) that circumvents the essentiality of mitochondrial gene expression. MGA KRGG1 CN cells cultured in the absence of tet (gray) grow indistinguishably from those with tet (black). (Inset) Western blot shows KRGG1 repression in −tet culture is maintained throughout the time course.
