Comparisons of KRGG1 and RESC proteins. (A) Schematic diagram of full-length T. brucei KRGG1 (cyan) and RESC6 (gray) sequences from 29.13 strain with amino-terminal region RGG and RG sequences highlighted in purple and blue, respectively. The Alphafold predicted ARM/HEAT-like tandem helical repeat region is indicated by labeled brackets and the conserved ARR and LLLN regions are indicated in red and magenta, respectively. Region of the schematic covered in the multispecies sequence alignment below indicated. (B) Similarity between RESC6 and KRGG1 amino acid sequences from eight kinetoplastid species. MUSCLE alignment shows conservation in the ARR (313–315) and LLLN (332–342) regions. One hundred percent similarity is shown as black, 80%–100% as dark gray, 60%–80% as light gray and <60% as white. (C) Alphafold predicted structures from T. brucei for KRGG1 (cyan), RESC6 (gray), and RESC8 (orange). Low confidence predictions of amino- and carboxy-terminal portions of KRGG1 (including the RGG repeat domain), RESC6, and RESC8 structures are not shown.
