FIGURE 4.
Identification of possible additional CFPS-limiting factors. (A) Sucrose gradient sedimentation analysis reveals the presence of disomes and trisomes in the CFPS system based on the extract from the engineered HEK293T cell line, independent of exogenously added mRNA. (B) Phosphorylation of eEF2 on T56 in the different translation systems. The capped HBB and uncapped EMCV IRES-containing polyadenylated mRNAs were used to drive the synthesis of nLuc. The gels are representative of two independent experiments.
