Depletion of mt-RNAs in direct RNA-seq of mouse heart tissue samples. (A) Schematic representation of the experimental setup. (Upper panel) Libraries were sequenced on a MinION flow cell, with 50% percent of channels in NS mode and 50% of channels in AS mode. (Lower panel) Reads were split prior to further analysis according to the AS decision. (B) Stack plot of reads per chromosome split according to the AS decision. (C) The Enrichment factor (per sequenced bases) for every chromosome was calculated for two biological replicates from reads split into “normal sequencing” and “total reads from adaptive sampling.” (D) Percent of sequenced bases mapping to the mitochondrial and nuclear chromosomes in AS and NS. (E) Length of reads mapping to the individual mitochondrial transcripts in AS and NS. (F) Normalized coverage (autoscale group to [0–18,974]) of reads mapped to the mitochondrial chromosome (chrM) in the individual samples as indicated. (G,H) Normalized coverage (autoscale group to [0–10]) of Ccl7 (G) and Rbm6 (H) in NS and AS as indicated.
