Quantification of elongation stalls and impact on gene expression in yeast

Dhh1 deletion, but not Hel2 deletion, affects gene expression in substitution constructs. (A) Protein expression of distributed stall constructs in dhh1Δ or hel2Δ background versus WT (n = 5, 4, 5, 6, 7, and 3 from left to right). (B) mRNA level fold change of distributed stall constructs in a dhh1Δ versus WT background (n = 5, 5, and 7 from left to right). (C) Elongation delay of distributed stall constructs in dhh1Δ or hel2Δ background versus WT (n = 19, 4, 18, 6, 9, and 5 from left to right). All error bars indicate SEM. All statistical significances were calculated for each construct using two-tailed paired Student's t-test against WT control.