Quantification of elongation stalls and impact on gene expression in yeast

Hel2 deletion rescues protein expression, mRNA levels, and elongation time. (A) Protein expression fold change of CGA constructs in a hel2Δ versus WT background (2×CGA n = 2, 4×CGA n = 7, 6×CGA n = 7). (B) mRNA level fold change of CGA constructs in a hel2Δ versus WT background (n = 3). (C) Elongation delay of CGA constructs in a hel2Δ versus WT background (n = 3). All error bars indicate SEM. All statistical significances were calculated for each construct using two-tailed paired Student's t-test against WT control.