Quantification of elongation stalls and impact on gene expression in yeast

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 1.
FIGURE 1.

Assay validation via elongation rate measurements. (A) Diagram of yeast-optimized constructs of various lengths. optYFP or both optYFP and optimized miRFP (miRFP) are set upstream of an nLuc reporter. Constructs are expressed from an inducible Tet07 promoter. (B, top) Representative assay data of relative light units (RLU) of each construct over time normalized to OD600. (Bottom) Schleif plot and associated trendlines of the top graph. (C) Calculated elongation rate measurements of optYFP (n = 9) and miRFP (n = 4) ORFs. Error bars indicate SEM.

This Article

  1. Published in Advance October 2, 2023, doi: 10.1261/rna.079663.123 RNA 29: 1928-1938

Current Issue

  1. July 2026, 32 (7)
  1. Current Issue
  1. Alert me to new issues of RNA